mouse secondary antibody (Vector Laboratories)

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Vector Laboratories mouse secondary antibody
Mouse Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1952 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horse+anti+mouse+igg+secondary+antibody/HRP+Horse+Anti-Mouse+IgG+Antibody+(Peroxidase)/pmc13050508-121-9-12
Average 96 stars, based on 1952 article reviews

mouse secondary antibody - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Harnessing the Plasma Proteome to Mirror Current and Predict Future Cardiac Remodeling After Myocardial Infarction.
Article Snippet: For IL-17E/IL-25 primary antibody, the blot was incubated with goat anti-rat IgG secondary antibody (Vector Laboratories, Cat. No. PI-9400, 1:5000). .. For the TIMP-1 primary antibody, the blot was incubated with horse anti-mouse IgG secondary antibody (Vector laboratories, Cat. No. PI-2000, 1:5000). .. Chemiluminescent images were captured using the iBright FL1000 imaging system (Thermo Fisher) and quantified using iBright analysis software 4.0.0.

Article Title: Chondrogenic differentiation of stem cells in human umbilical cord stroma with PGA and PLLA scaffolds
Article Snippet: .. Then the sections were incubated with a streptavidin-linked horse anti-mouse IgG secondary antibody (Vector Laboratories, Burlingame, CA). .. After secondary antibody incubation, the sections were incubated with an avidin-biotinylated enzyme complex (ABC complex; Vector Laboratories) and VIP substrate (Vector Laboratories).

Article Title: Idiopathic pulmonary fibrosis is associated with tight junction protein alterations.
Article Snippet: .. For these cases, after antigen retrieval, tissue sections were quenching in 3% hydrogen peroxide (ThermoFisher Scientific, H325-100) in deionized water, blocked for 20 minutes with 2.5% normal horse serum, incubated with primary antibodies for 60 minutes (1:200 for ZO-1; 1:100 for claudin-5; 1:50 for claudin-2), washed and incubated with horse anti-mouse IgG secondary antibody for 30 minutes (Vector Laboratories, MP-7401). .. Antigen-antibody binding was detected using ImmPACT® DAB Peroxidase (HRP) substrate kit (Vector Laboratories, SK-4105).

Article Title: Harnessing the Plasma Proteome to Mirror Current and Predict Future Cardiac Remodeling After Myocardial Infarction
Article Snippet: For IL-17E/IL-25 primary antibody, the blot was incubated with goat anti-rat IgG secondary antibody (Vector Laboratories, Cat. No. PI-9400, 1:5000). .. For the TIMP-1 primary antibody, the blot was incubated with horse anti-mouse IgG secondary antibody (Vector laboratories, Cat. No. PI-2000, 1:5000). .. Chemiluminescent images were captured using the iBright FL1000 imaging system (Thermo Fisher) and quantified using iBright analysis software 4.0.0.

Protease Inhibitor:

Article Title: Investigation of estrogen-like effects of parabens on human neutrophils.
Article Snippet: .. 2,0, Latex Beads Polystyrene, Griess’ reagent for nitrite, Superoxide dismutase, N-Formylmethionineleucyl-phenylalanine, NBT Nitrotetrazolium Blue Chloride, Penicillin and Streptomycin antibiotic, Protease Inhibitor Cocktail (Merck Millipore, Burlington, USA), Anti-ERα (F-10): sc-8002 antibody, Anti-ERβ (B1): sc-390243 antibody, Anti-Neutrophil Elastase (G-2): sc-55549 antibody, Anti-Proteinase 3 (D-1): sc-74534 antibody, Anti-β-Actin (9): sc130301 antibody, ERβ receptor antagonist - PHTPP (Santa Cruz Biotechnology, Dallas, USA), GPER receptor antagonist - G-15 (Tocris Bioscience, Bristol, UK), Horse Anti-Mouse IgG secondary antibody (Vector Laboratories, Inc., Burlingame, USA), Mouse Anti-Goat IgG secondary antibody (Jackson ImmunoResearch, Cambridgeshire, UK), Vybrant® MTT Cell Proliferation Assay Kit, QubitTM Protein Assay Kit, Hoechst 33342 Fluorescent Nucleic Acid Stain, Myeloperoxidase Monoclonal Antibody (8E6)-FITC (Invitrogen, Waltham, USA), Annexin V-APC, BDTM Sheath Additive, 7-AAD (BD Biosciences, Jersey, USA), May-Grunwald and Giemsa solutions (Aqua Med, Łódź, Poland), RPMI1640 with L-glutamine and NaHCO3 (Biomed-Lublin, Lublin, Poland), PolymorphprepTM (Axis-Shield, Dundee, UK), MACS Separation Columns LS, CD16 MicroBeads Human and AutoMACS Running Buffer (Miltenyi Biotec, Bergisch Gladbach, Germany), Filters for Boyden Chamber, Boyden Chamber (Neuro Probe, Inc., Gaithersburg, USA), 3- Color Prestained Protein Marker (DNA Gdansk, Gdansk, Poland), Vacuette NH Sodium Heparin (Greiner Bio-One GmbH, Kremsmünster, Austria), 4 × Laemmli Sample Buffer, 2-mercaptoethanol, Mini TransBlot® Filter Paper, Ammonium Persulfate (APS), 10x Tris/Glycine Buffer, 10x Tris/Glycine/SDS, Stacking Gel Buffer for PAGE 0.5 M TrisHCl, pH 6.8, Resolving Gel Buffer for PAGE, Tween® 20, 1xTBS 1% Casein Blocker (Bio-Rad Laboratories, Inc., Hercules, USA), FSC (PANBiotech GmbH, Aidenbach, Germany), Trypan blue solution (Lachema, Praga, Czech Republic). ..

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Article Title: Intra-articular etanercept attenuates pain and hypoxia from TMJ loading in the rat.
Article Snippet: Mechanical overloading of the temporomandibular joint (TMJ) and biochemical changes, like inflammation and hypoxia, contribute to cartilage degeneration and pain associated with osteoarthritis (OA).. Yet, how overloading contributes to early dysregulation of chondrocytes is not understood, limiting the development of diagnostics and treatments for TMJ OA.. Hypoxia inducible factors (HIF)-1α/2α in chondrocytes were evaluated at days 8 and 15 in a rat TMJ pain model induced by jaw loading (1hr/day for 7 days) using immunohistochemistry and compared between cases that induce persistent (3.5N), acute (2N), or no (0N) sensitivity.

Article Title: Intraarticular etanercept attenuates pain and hypoxia from TMJ loading in the rat
Article Snippet: Sections were blocked for 2hrs at room temperature with 10% normal horse serum (Abcam; Cambridge, MA) and 0.3% Triton-X (Bio-Rad; Hercules, CA) and incubated overnight at 4°C with primary antibodies for rabbit anti-HIF-1α (1:500; Abcam), mouse anti-HIF-2α (1:500, Abcam), rabbit anti-TNF-α (1:500; ABD Serotec; Raleigh, NC), or murine ELK3–51 monoclonal antibodies (75μg/ml; Hypoxia Imaging Service Center at the University of Pennsylvania). .. Slides were washed with PBS and labeled with biotinylated horse anti-rabbit or horse anti-mouse IgG secondary antibody (1:1,000; Vector; Burlingame, CA) for 2hrs at room temperature. .. After washing with PBS, slides were incubated in avidin-biotin complex reagent (Vector), developed using a 3,3-diamimobenzidine kit (DAB; Vector), and cover slipped using DPX mounting medium (Millipore Sigma; St. Louis, MO).

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